真核生物转录调控概念，策略和方法

出版时间：2002-01-01  出版社：清华大学出版社  作者：(美)Michael Carey  

内容概要

本书描述了真核生物的基因转录调控过程中的分子机理，分析启动子和转录因子以及其他与真核基因转录调控有关的研究路线和实验技术。

书籍目录

1 A PRIMER ON TRANSCRIPTIONAL REGULATION IN MAMMALIAN CELLS
2 INITIAL STRATEGIC ISSUES
3 MODES OF REGULATING mRNA ABUNDANCE
4 TRANSCRIPTION INTIATION SITE MAPPING
5 FUNCTIONAL ASSAYS FOR PROMOTER ANALYSIS
6 IDENTIFICATION AND ANALYSIS OF DISTANT CONTROL REGIONS
7 IDENTIFYING cis-ACTING DNA ELEMENTS WITHIN A CONTROL REGI
N_8 IDENTIFICATION OF DNA-BINDING PROTEINS AND ISOLATION OF THEIR GENES
9 CONFIRMING THE FUNCTIONAL IMPORTANCE OF A PROTEIN-DNA INTERACTI
N_10 IN VIVO ANALYSIS OF AN ENDOGENOUS CONTROL REGI
N_11 APPROACHES FOR THE SYNTHESIS OF RECOMBINANT TRANSCRIPTION FACTORS
12 IDENTIFYING AND CHARACTERIZING TRANSCRIPTION FACTOR DOMAINS
13 THEORY，CHARACTERIZATION，AND MODELING OF DNA BINDING BY REGULATORY TRANSCRIPTION FACTORS
14 CRUDE AND FRACTIONATED SYSTEMS FOR IN VITRO TRANSCRIPTI
N_15 APPROACHES FOR STUDYING TRANSCRIPTION COMPLEX ASSEMBLY
APPENDICES
INDEX