临床生物化学实用教程

出版时间：2003-6  出版社：北京世界图书出版公司  作者：J.S.Varcoe  

内容概要

本书是一部注重理论和和实践结合的临床生化教程。作者认为亲自动手去做实验会极大地增强人们对分析技术和所采用仪器的理解，因此，本书作者力求覆盖在临床生物化学中使用的主要分析技术和仪器，分析技术主要包括色谱学、光学、电化学、电泳、酶学、免疫学、分子诊断学和其它一些技术，并附以练习帮助学生对于仪器分析应用的理解。要目：分光光度；X光检测法；酶学；原子泄出和吸收；离子选电极；色谱法；薄层色谱法和提取技术；气体色谱法；高性能液态色谱法；电泳法；分子诊断学；免疫学技术和X射线检测法。读者对象：临床生物化学和相关专业的本科生及研究生。

书籍目录

AcknowledgmentsAbbreviations.Chapter 1. Spectrophotometry  Photometric Analysis.    The Effect of Solvent 1 5  Laws of Radiant Energy Absorption.    Lambert's Law 1-6: Beer's Law 1-6; Beer Lambert's Law 1-8,    Interrelationship Between Absorbance and Transmittance I 8  Colour and Colorimeters.  The Blank.  Instrumentation.    Sources of Radiant Energy 1-9; Means of Spectral isolation 1 11;    Adjustable Diaphragm 1-15; Cuvettes 1-15; Radiant Energy Detectors 1-16; Readout Systems 1-21; A Typical Single Beam Speetrophotometer 1-21; Double Beam lnstruments 1-22; A Typical Double Beam up" Period 1 25; Optimum Spectral Band Width 1-25; Stray Light 1 27;Correction for BackRround Absorption 1-30   Checking Instrument Performance.    Wavelength Alignment I 33: Wavelength Repeatability 1-34; Spectral BandWidth 1-34; StrayLight 135:Pbotometric Aecuracy and Linearity 1-35  Exercises.    Exercise 1-1: Photometric Accuracy and Linearity.    Exercise 1-2: Spectral Band Width.    Exercise 1-3: Spectral Characteristics of a Filter.    Exercise 1-4: Wavelength Check.    Exercise 1-5: Spectral Band Width and Resolution.    Exercise 1-6: Cuvette Cut-Off Wavelengths.    Exercise 1-7: The Absorption Spectrum of Benzene.    Exercise 1-8: The Absorption Spectra of NAD and NADH,    Exercise 1-9: Stray Light.    Exercise 1-10: Derivative Spectra.    Exercise 1-11: The Determination of Serum Calcium.    Exercise 1-12: The Determination of Carbon Monoxide in Blood.    Exercise 1-13: The Determination of Plasma SaEey[ate.    Exercise 1-14: The Determination of Plasma ParacetamobChapter 2.  Fluorimetry  Fluorescence,    Factors Affecting Fluorescence Intensity : Concentration 2-3, Solvent 2 4,    Buffers 2-5,  pH and lonisation 2-5,  Temperature 2-6,  Photo-decomposition 2-6, Quenching 2-6 instrumentation,    Light Sources 2 8; Sample Containers 2-9; Detectors 2-9; Sensitivity 2-9; An Example of a Spectrofluorimeter 2 10  Exercises    Exercise 2-1 : The Effect of Concentration on Fluorescence Intensity.    Exercise 2-2 : ]he Effect of Solvent on Fluorescence Intensity.    Exercise 2-3 : The Effect of pH on Fluorescence Intensity.    Exercise 2-4 : The Effect of Temperature on Fluorescence Intensity.    Exercise 2-5 : The Effect of Quenching on Fluorescence intensity.    Exercise 2-6 : The Emission Spectrum of NADtt.    Exercise 2 7 : The Determination of Thiamine in Urine.    Exercise 2-8 : The Determination of Serum Magnesium.    Exercise 2 9 : The Determination of Phenylalanine in Plasma.    Exercise 2 10 : The Determination of Blood Porphyrins.    ReferencesChapter 3.Enzymology.  Enzymes.    Catalysis 3-1; Classiflcation and Nomenclature 3-2; EnzymeDnits 3 3:Modes of Action 3-4; The Active Site 3-4: Substrate Specificity 3-4; pH3-5; Temperature 3-5; Enzyme Kinetics 3-6; Enzyme Inhibitlon 3-9: Allosteric Enzymes 3-12; Isoenzymes 3-13  Clinical Enzymology.    The Diagnostic Use of lsoenzymes 3-17  Enzymes as Reagents.  Exercises.    Exercise 3-1: The Polarimetric Determhlatlon of tnvertase.    Exercise 3-2: The Effect of Temperature on lnvertase Activity.    Exercise 3-3: The Effect of pH on [nvertase Activity.    Exercise 3-4: The Effect of Substrate on Yeast Alcohol Dehydrogenase Activity,    Exercise 3-5 : The Effect of Substrate Concentration on Yeast Alcohol Dehydrogenase Activity.    Exercise 3-6 : The Effect of an Inhibitor on Yeast Alcohol Dehydrogenase Activity.    Exercise 3-7 : The Determination of Prostatic Acid Phosphatase in Plasma.    Exercise 3-8 : The Determination of Plasma Lactate Dehydrogenase and its lsoenzymes,    Exercise 3 9 : The Fluorimetric Determination of Alkaline Fhosphatase in Plasma.……Chapter4. Atomic Emission and AbsorptinChapter5. Ion Selective Electrodes.Chapter6. Oxygen and Carbon Dixide Electrodes.Chapter7. Chromatography.Chapter8. Thin Layer Chromatography and Extraction Techniques.Chapter9. Gas Chromatography.Chapter10. High Performance Liquid Chromatography.Chapter11. Electophoresis.Chapter12. Molecular Diagnostics.Chapter13. Immunological and Radioisotope Techniques.Chapter14. Coulometry,Osmometry and RefractometryAppendicesIndex